Comparison of phi29-based whole genome amplification and whole transcriptome amplification in dengue virus
نویسندگان
چکیده
منابع مشابه
Primase-based whole genome amplification
In vitro DNA amplification methods, such as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction. In vivo, primers are synthesized on-template by DNA primase. The bacteriophage T7 gene 4 protein (gp4) has both primase and helicase activities. In this study, we report the development of a primase-based Whole Genome Amplification (pWGA) method,...
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Major efforts are underway to systematically define the somatic and germline genetic variations causally associated with disease. Genome-wide genetic analysis of actual clinical samples is, however, limited by the paucity of genomic DNA available. Here we have tested the fidelity and genome representation of phi29 polymerase-based genome amplification (phi29MDA) using direct sequencing and high...
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INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. In contrast to other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable t...
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Single-cell sequencing is emerging as an important tool for studies of genomic heterogeneity. Whole genome amplification (WGA) is a key step in single-cell sequencing workflows and a multitude of methods have been introduced. Here, we compare three state-of-the-art methods on both bulk and single-cell samples of E. coli DNA: Multiple Displacement Amplification (MDA), Multiple Annealing and Loop...
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Whole genome amplification (WGA) plays an important role in sample preparation of low-input templates for high-throughput sequencing. Multiple displacement amplification (MDA), a popular isothermal WGA methods, suffers a major hurdle of highly uneven amplification. Optimizations have been made in the past by separating the reagents into numbers of tiny chambers or droplets in microfluidic devic...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2014
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2013.10.005